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2022 Vertebrate eDNA

CalCOFI October 2022 Vertebrate eDNA

calcofi_2022-ednaCalCOFICC BY 4.0DOI 10.15468/n52j6rin the release since v2026.10.05validated

eDNA metabarcoding survey of vertebrates from water samples collected during the October 2022 CalCOFI cruise. CalCOFI holds 148 observations from 47 sampling events at 13 stations, 2022, 5–380 m.

2022
years
148
observations
47
sampling events
13
stations
12
species
5–380 m
depth
What this is

eDNA metabarcoding survey of vertebrates from water samples collected during the October 2022 CalCOFI cruise. Water filters from CalCOFI/GEMCAP stations off Southern California were run through one or two molecular assays: a mitochondrial D-loop assay (cetacean detections, incl. Delphinus and Megaptera novaeangliae) and a 12S rRNA “MiFish” assay (fish detections). Published as an intercalibration exercise (source datasetID “CalCOFI_Intercal”) on GBIF/OBIS, linked to Patin et al. 2026 (Methods in Ecology and Evolution, https://doi.org/10.1111/2041-210x.70370). Sequence read counts are a semi-quantitative signal of PCR-amplified DNA, not abundance, and are not comparable between assays. Published as detections only: edna_presence = 1 and the read counts. The source archive holds positive detections only, so the absence of a row for a filter and taxon is not a non-detection for this dataset (a filter or assay run with no detection is simply missing, and absence cannot be inferred); this differs from sio_cetacean-edna, where 0 means not detected in a sequenced sample.

Principal investigators
Nastassia Patin
Creators
Nastassia Patin (Scripps Institution of Oceanography, UC San Diego) · Garret O'Donnell (Scripps - California Cooperative Oceanic Fisheries Investigations)
Funding
ONR MURI N00014-22-1-2719; NSF OCE-2224726
Genomics & eDNA
Metabarcoding and environmental DNA — ASV tables, eDNA-derived abundance and density
st55-ln66.7 · 10 obs · 2022–2022st60-ln70 · 4 obs · 2022–2022st70-ln70 · 2 obs · 2022–2022st50-ln73.3 · 8 obs · 2022–2022st55-ln73.3 · 2 obs · 2022–2022st60-ln73.3 · 4 obs · 2022–2022st51-ln76.7 · 16 obs · 2022–2022st55-ln76.7 · 6 obs · 2022–2022st55-ln80 · 16 obs · 2022–2022st60-ln80 · 4 obs · 2022–2022st46.9-ln81.8 · 38 obs · 2022–2022st42-ln83.3 · 34 obs · 2022–2022st51-ln83.3 · 4 obs · 2022–2022125°W120°W30°N35°N
13 CalCOFI stations sampled, sized by observations, over the survey grid.

What is in it

2022 · 148 obs19501960197019801990200020102020
Observations per year, 2022–2022.
17 variables · edna_presence, edna_reads_12s, edna_reads_dloop, ammonia, nitrate, nitrite, oxygen_mg_l, phosphate, silicate, chl_fluor … all 17

obs_bio profile · one value per taxon at a sampling event · 148 values · 2022 · 3 variables

edna_presence dimensionless 74edna_reads_12s reads 23edna_reads_dloop reads 51

sample_measurement per cast · one value for the whole cast or tow, at no depth · 536 values on 47 sampling events · 2022 · 14 variables

ammonia umol/L 47nitrate umol/L 47nitrite umol/L 47oxygen_mg_l mg/L 47phosphate umol/L 47silicate umol/L 47chl_fluor ug/L 46dna_concentration ng/uL 46edna_asvs_dloop count 37edna_reads_filtered_dloop reads 37edna_reads_raw_dloop reads 37edna_asvs_12s count 17edna_reads_filtered_12s reads 17edna_reads_raw_12s reads 17

19 taxa · Delphinus delphis short-beaked common dolphin, Delphinus common dolphins, Engraulis mordax northern anchovy, Leuroglossus, Cololabis saira pacific saury, Coryphaena hippurus common dolphinfish … the 19 most observed

1 of 12 months · extent 33.8–36.3°N, 122.8–119.4°W · 2022-10 to 2022-10

Ways in

Explorerdb-queryOriginal at GBIFParquetSTACIngest notebookAs JSON

Every endpoint 23 · explore 4, get the data 3, code 5, metadata records 5, archives & portals 6

Every endpoint this dataset can be reached through, grouped by how you would use it. Each name is the link; the copy button beside it copies the address. Everything is in the release record and was answered when the release was cut.

Apps that read this dataset from the release. The icons after a name are the app’s lenses — the spatial grain it shows the data at.

CalCOFI ExplorerAcross datasetsopens on this dataset
Station ExplorerAcross datasetsopens on this dataset
Hexagon ExplorerAcross datasetsopens on this dataset
Cruise ExplorerAcross datasetsopens on this dataset
Cite Patin N, ODonnell G, 2026 · DOI

This dataset's own citation. What to cite, and how, is a chapter of the docs book.

Patin N, ODonnell G (2026). CalCOFI October 2022 Vertebrate eDNA. Version 1.3. Occurrence dataset. https://doi.org/10.15468/n52j6r

License: CC-BY-4.0
DOI: https://doi.org/10.15468/n52j6r
Acknowledgement: This work was made possible by the Office of Naval Research Multidisciplinary University Research Initiative (MURI) funding for MMARINeDNA (award no.: N00014-22-1-2719) with additional support from the National Science Foundation (award no.: OCE-2224726). It includes data generated at the UC San Diego IGM Genomics Center utilizing an Illumina NovaSeq 6000 that was purchased with funding from a National Institutes of Health SIG grant (no.: S10 OD026929). We thank the captain and crew of the NOAA Ship Reuben Lasker, Rachel Pound from CalCOFI and Harrison Huang and Dylan Inskeep from the California Department of Fish and Wildlife for their assistance with sample collection.
BibTeX
@misc{calcofi_2022-edna,
  title        = {CalCOFI October 2022 Vertebrate eDNA},
  howpublished = {Patin N, ODonnell G (2026). CalCOFI October 2022 Vertebrate eDNA. Version 1.3. Occurrence dataset. https://doi.org/10.15468/n52j6r
},
  year         = {2026},
  doi          = {10.15468/n52j6r},
  url          = {https://doi.org/10.15468/n52j6r},
  note         = {License: CC-BY-4.0; Acknowledgement: This work was made possible by the Office of Naval Research Multidisciplinary University Research Initiative (MURI) funding for MMARINeDNA (award no.: N00014-22-1-2719) with additional support from the National Science Foundation (award no.: OCE-2224726). It includes data generated at the UC San Diego IGM Genomics Center utilizing an Illumina NovaSeq 6000 that was purchased with funding from a National Institutes of Health SIG grant (no.: S10 OD026929). We thank the captain and crew of the NOAA Ship Reuben Lasker, Rachel Pound from CalCOFI and Harrison Huang and Dylan Inskeep from the California Department of Fish and Wildlife for their assistance with sample collection.
}
}

Acknowledgement. This work was made possible by the Office of Naval Research Multidisciplinary University Research Initiative (MURI) funding for MMARINeDNA (award no.: N00014-22-1-2719) with additional support from the National Science Foundation (award no.: OCE-2224726). It includes data generated at the UC San Diego IGM Genomics Center utilizing an Illumina NovaSeq 6000 that was purchased with funding from a National Institutes of Health SIG grant (no.: S10 OD026929). We thank the captain and crew of the NOAA Ship Reuben Lasker, Rachel Pound from CalCOFI and Harrison Huang and Dylan Inskeep from the California Department of Fish and Wildlife for their assistance with sample collection.

…and the release it came from

CalCOFI (2026). CalCOFI Integrated Database, release v2026.10.21 [Data set]. Scripps Institution of Oceanography, NOAA Fisheries, and California Department of Fish and Wildlife. https://calcofi.io/db-schema/?v=v2026.10.21
BibTeX
@misc{calcofi_release_v2026_10_21,
  title     = {CalCOFI Integrated Database, release v2026.10.21},
  author    = {CalCOFI},
  year      = {2026},
  publisher = {Scripps Institution of Oceanography, NOAA Fisheries, and California Department of Fish and Wildlife},
  url       = {https://calcofi.io/db-schema/?v=v2026.10.21}
}
Provenance source read 2026-10-21 · 6 release tables · 2 changed in v2026.10.21 · 4 source files
source accessed
2026-10-21
record updated
2026-10-01
blockers
Un-held 2026-10-04 (Ben): ships in the next release as detections only (edna_presence = 1 and read counts; the archive holds positive detections only, so no row is not a non-detection). Q08 (omitted zero-detection runs) stays open, now high; Q07 (read filtering, controls) and Q06 (ASV/PCR-replicate summing) stay open. 2026-10-01 rework of PR #118 (review decision B4): obs = one row per filter x taxon x measurement_type -- edna_presence (shared with sio_cetacean-edna; 1 only, positive detections) + edna_reads_dloop / edna_reads_12s (reads summed over ASVs and PCR replicates); no obs_attribute; sample_key calcofi_2022-edna:filter:<parentEventID>; sample_measurement carries nutrients/chl_fluor/oxygen_mg_l/dna_concentration + per-assay raw and filtered read totals; 'OTUs or ASVs assigned to taxa' held back (Q04: equals the summed reads). Rendered + uploaded to gs://calcofi-db/ingest/calcofi_2022-edna/ 2026-10-01: sample 47 / obs 148 / sample_measurement 482. site_key/parent_sample_key NULL pending Q05.

What changed in v2026.10.21

Source files

The original inputs the ingest notebook read, archived by the ingest to the public files bucket — 4 files, 378 KB.

release changelog ↗

Keywords 4 GCMD

eDNAmetabarcodingdolphinsrare targets

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